Dockerin is a protein domain found in the cellulosome cellular structure of anaerobic bacteria. It is found on many endoglucanase enzymes. The dockerin's binding partner is the cohesin domain, located on the scaffoldin protein. This interaction between the dockerin domains of the enzyme constituents of the cellulosome and the cohesin domains of the scaffoldin protein is essential to t… WebMay 1, 2008 · Many investigations were conducted to characterize the cohesin-dockerin interactions. These studies include measuring cohesin-dockerin affinity, identifying …
纤维素酶及其基因结构特征与功能的关系 - 豆丁网
WebFeb 27, 2007 · Clostridium thermocellum type I dockerin modules contain a duplicated 22-residue sequence that comprises helix-1 and helix-3, respectively. The crystal structure … WebIn any case, the cohesin-dockerin complex in the C. thermocellum system represents one of the highest protein-protein affinity interactions known in nature, rivaling many other … braewood townhomes
Cohesin-Dockerin Interactions and Folding - Wiley Online Library
WebJan 12, 2024 · Cellulosomal enzymes are bound to a non-enzymatic membrane-anchoring protein, a scaffoldin, via a modular dockerin domain attached to the enzymes which interact and bind with cohesion proteins on the scaffoldin [60, 61], thus creating dynamic and powerful catabolic complexes (see Fig. 2). WebDec 7, 1998 · Cohesin-containing segments from the cellulosomes of two different species, Clostridium thermocellum and Clostridium cellulolyticum, were allowed to interact with … Our work focuses on the molecular basis of the interaction between the binding domains of the cellulosome using biochemical and molecular biological techniques. We employed progressive deletion and alanine-scanning techniques on a representative type-I dockerin module from Clostridium … See more Cohesin modules are the major building blocks of scaffoldins which are responsible for organizing the cellulolytic subunits into the multi-enzyme … See more The dockerin module anchors the catalytic subunits to the scaffoldin. It displays internal two-fold symmetry, consisting of a duplicated F-hand motif (a calcium-binding loop preceding an … See more In this study, we characterized functionally and structurally a unique, atypical type-III dockerin from the Ruminococcus flavefaciens ScaA scaffoldin (ScaADoc). By using different spectroscopic measurements we … See more The cohesin-dockerin pair represents a high-affinity (Kd 10-9-10-12M) protein-protein interaction. This interaction tends to be nonspecific within a given species but specific between species. Thus, a scaffoldin-borne … See more hackers arise hackrf one