Cyto-id® green detection reagent

Author: ekqk

August undefined, 2024

WebDec 22, 2024 · The cell residues were washed twice with the assay buffer, and 100 μl of Dual Detection Reagent (2 μl of Cyto-ID Green Detection Reagent and 1 μl of Hoechst 33,342 Nuclear Stain in 1 mL of cell culture medium) was then dispensed in each well. The cells were protected from light and incubated at 37°C for 30 min. WebJan 16, 2016 · Cyto-ID Green concentration contains 1 μl of Cyto-ID Green Detection Reagent in 1 ml cell culture medium. It was then mixed well and incubated for 30 min under standard tissue culture conditions at 37 °C, 5 % CO 2 in the dark. At the end of staining procedure, the Cyto-ID containing medium was washed with PBS.

Detection of Paraptosis After Photodynamic Therapy

WebNUCLEAR-ID ® Green chromatin condensation detection kit : A highly permeable green-emitting dye for enhanced detection of apoptosis … the pinvin federation

Enzo Life Sciences CYTO-ID Autophagy detection kit …

WebIncase of Low CYTO-ID Green dye staining in all treatments, increase the reagent concentration (500X dilution of the dye is recommended) and the incubation time. ... grow the cells on coverslips after respective treatment incubate them with the dual detection reagent (Cyto‐ID dye and Hoechst 3342 nuclear stain) at 37 ºC for 30 minutes in the ... http://cdn035.yun-img.com/static/upload/yjinbio/team/20240502141735_89957.pdf Web1. Product and Company Identification Product Code: 20-2958-xx Product Name: 3'-Amino-Modifier C7 CPG 1000 Product Use: For Research Use Only Manufacturer Information Company Name: Glen Research LLC 22825 Davis Drive Sterling, VA 20164 USA Phone Number: 1-703-437-6191 Web site address: www.glenresearch.com Email address: … the pinwheel

Cells Free Full-Text Substrate Reduction Therapy Reverses

Using Cellometer Vision and Cyto-ID Stain for Autophagy …

WebCYTO-ID® Autophagy Detection Kit 10662-266 / 89165-926 Dyes, Rapamycin, Chloroquine, 10x assay buffer CYTO-ID® Autophagy Detection Kit 2.0 75802-912 / 75802-914 Dyes, Rapamycin, Chloroquine, 10x assay buffer Dyes Included in Kit Excitation/Emission Platform Cell Type CYTO-ID® Green Detection Reagent Hoechst … WebCYTO-ID Autophagy detection kit (1x200tests) A no-transfection, quantitative assay for monitoring autophagy in live cells. CYTO-ID.The probe is a cationic amphiphilic tracer … the pinwheel doylestown paWebAug 6, 2024 · Autophagy analysis was performed by incubating cells with bafilomycin A1 for 30 min at 37 °C prior to treatment with CYTO-ID Green Detection Reagent, and then analyzing fluorescence by flow cytometry using the … side effects of bone strengthening meds

"WebCyto-ID labeling and Flow cytometry: HEK-293 cells were harvested in Accutase solution (Merck Millipore, SF006), rinsed in DPBS and incubated for 30 min at room temperature … " - Cyto-id® green detection reagent

Cyto-id® green detection reagent

ELISA Reader Machines & Equipment Dynex Technologies

WebFluorescent detection, HTS. Contents: CYTO-ID Green Detection Reagent. Hoechst 33342 Nuclear Stain. Autophagy Inducer (Rapamycin). Chloroquine Control. 10X Assay Buffer Catalog No. 50-103-1813 $558.00 / Each Qty Check Availability Add to cart Provide Content Correction WebJun 13, 2024 · Inflorescence-based of CYTO-ID® autophagy detection. Primary chondrocytes isolated from the cartilage of WT and GRN −/− mice were treated with DMSO (control) or IL-1β (1 μM) for 24 h, then stained with CYTO-ID® Green Detection Reagent and LysoTracker Red at a concentration of 300 nM was added and incubated at 37°C for …

Did you know?

WebOct 1, 2024 · The autophagy level of haemocytes was detected with CYTO-ID® Autophagy Detection Kit (Enzo, Switzerland) according to the instruction. ... for 3 h, and then washed two times with M-L15 medium. The haemocytes on the slide were incubated with 200 μL CYTO-ID® Green Detection Reagent (diluted 1:500 in M-L15 medium) at 37 °C in dark … WebDec 1, 2024 · CYTO-ID® Green dye eliminates background staining of lysosomes seen with other lysosomotrophic dye-based assays that utilize monodansylcadaverine (MDC) (bottom panel). The CYTO-ID® …

WebThe Cyto-ID™ Autophagy Detection Kit is a member of the CELLestial® product line, reagents and assay kits comprising fluorescent molecular probes that have been … WebReagents Hypoxia Red Detection Reagent, part of the Cyto-ID® Hypoxia/Oxidative Stress Detection Kit (Catalog No. ENZ-51042) was provided by Enzo Life Sciences (Farmingdale, NY). The reagent is a weakly-fluorescent compound containing a nitro (NO 2) moiety. Due to nitroreductase activity present in hypoxic

WebFeb 1, 2024 · After 24 h of laser irradiation, cells were labeled with CYTO-ID® Green. All the fluorescent images were captured using Cytation5 cell imaging multi-mode reader with objective lens 20×, followed by uniform processing using Cytation5 software (bar = 100 μm). ... To assess autophagy, we used CYTO-ID® Autophagy Detection Kit 2.0 based on a … WebProtocol tips Dilute 4 μl of Cyto-ID® Green Detection Reagent in 1ml of diluted assay buffer and stain the cells for 30 min at 37°C in the dark. Publication protocol Macrophage infection assay

WebProduct overview. Autophagy Assay Kit ab139484 measures autophagic vacuoles and monitors autophagic flux in live cells using a dye that selectively labels autophagic …

http://biopioneer.com.tw/?p=41098 side effects of bone upWebMay 4, 2024 · The pattern of vacuole labeling 4 h after ER photodamage in the presence vs. absence of 5 μM cycloheximide with ERTracker Green, a fluorescent probe for the ER, is … side effects of bone marrow testWebThe green dye emits in the green region of the visible light spectrum and is thus not compatible with GFP. b) Flow cytometry application: Make a dye stain solution by diluting 1 μL Green Detection Reagent to 1 mL cell culture medium without Phenol Red Indicator, supplemented with 5% FBS. For the pinwheel fishing boatWebOct 20, 2016 · The Cyto-ID ® Green Reagent is a cationic amphiphilic tracer dye which selectively labels pre-autophagosomes, autophagosomes, and autophagolysosomes. … side effects of boost energy drinkWebFeb 14, 2024 · Cyto-ID green detection reagent, consisting of 1 μL Hoechst 33342, 100 μL Cyto-ID reagent per 1 mL cell culture medium supplemented with 10% FBS was used to stained cells. The plate was stored ... the pinwheel groupWebEnzo Life Sciences CYTO-ID Autophagy detection kit (1x50tests) A no-transfection, quantitative assay for monitoring autophagy in live cells. CYTO-ID.The probe is a cationic amphiphilic tracer (CAT) dye that rapidly … side effects of boostrixWebCyto-ID Green autophagy dye staining procedure for autophagy detection Cyto-ID Green solution was prepared by mixing the dye with 1× assay buffer. The cell sample was centrifuged, and the dye was pipetted in for image-based or flow cytometry, respectively. The sample was shielded from direct light and incubated for 30 min at 37°C, the pinwheel galaxy