WebJul 7, 2024 · Here, the authors use deep mutational scanning to identify mutations in the RBD that escape human monoclonal antibodies or convalescent plasmas. ... Hilton, S. et al. dms-view: interactive ... WebThe spike (S) protein of SARS-CoV-2, which is undergoing rapid evolution, plays crucial roles in viral immune escape, infectivity, and transmissibility. To gain clinical insight, Dadonaite et al. developed a novel deep mutational scanning (DMS) platform for mapping the effects of S protein mutations on immune evasion and viral infectivity.

Deep mutational scanning: a new style of protein science

WebMar 22, 2024 · The major concern with COVID-19 therapeutic monoclonal antibodies is the loss of efficacy against continuously emerging variants of SARS-CoV-2. To predict antibody efficacy against future Omicron subvariants, we conducted deep mutational scanning (DMS) encompassing all single mutations of the receptor-binding domain of the BA.2 … WebDeep mutational scanning experiments. Deep mutational scanning is a high-throughput functional assay that measures the effect of every genetic change at every site in a … goodrich reload

Deep mutational scanning for therapeutic antibody engineering

WebApr 10, 2024 · Designing vaccine immunogens is often a tedious process. Here the authors develop a deep mutational scanning-based method to rapidly and comprehensively identify prefusion stabilizing mutations of ... WebOct 13, 2024 · We carried out deep mutational scanning (DMS) of four homologous bacterial allosteric transcription factors (aTFs) to identify hotspots and built a machine learning model with this data to glean the structural and molecular properties of allosteric hotspots. We found hotspots to be distributed protein-wide rather than being restricted to ... WebMaveDB is a public repository for datasets from Multiplexed Assays of Variant Effect (MAVEs), such as those generated by deep mutational scanning (DMS) or massively … goodrich rebate