WebPhotocaged peptides and proteins represent one important tool in the toolbox of methods to build light-responsive complex ... ,67,69–75 serine (Ser),76 tyrosine (Tyr),77,78 aspartate (Asp),79 glutamate (Glu),80,81 asparagine (Asn),82 glutamine (Gln),83 and lysine (Lys)73,84,85 residues. Other photocaging agents have been less commonly ... WebOct 7, 2016 · Herein, we provide details on experimental design and the utilization of this photocaged intein to photoactivate the Src tyrosine kinase in human embryonic kidney …
Homology modeling of Subtilisin mutated with a photocaged …
WebWe have recently reported the direct light activation of TET dioxygenases in cells by the incorporation of 4,5-dimethoxy-2-nitrobenzyl-L-serine (1, Figure 3 a) via amber suppression. 18 We ... WebJan 30, 2014 · Several ncAAs including photocaged tyrosines (Hino et al. 2005;Valentin-Hansen et al. 2014;Murray et al. 2016), cysteines (Köhrer et al. 2004;Xiao et al. 2013; Nguyen et al. 2014; Zheng et al ... peter hofmann physioscheune
Site-Specific Incorporation of Selenocysteine by Genetic ... - PubMed
WebDec 18, 2024 · For proteins, systems for biosynthetic incorporation of photocaged lysine, tyrosine, serine, cysteine, and selenocysteine residues have been described (Riggsbee … WebAs serine (or cysteine) at position 1 of the intein is needed for the protein splicing pathway, the usage of a so-called photocaged serine – a serine with a light-removable protecting group (photolabile ‘caging’ group) attached to it – allowed inhibiting splicing by Tli pol-2 intein until UV light liberated the catalytic residue and ... WebJan 30, 2014 · Photocaged lysine, tyrosine, and serine have been site specifically incorporated in place of key residues, allowing photocontrol of nuclear localization sequences, kinases, and other enzymes, and photocontrol of the sites of post-translational modification. 2,4,8−11 These approaches have revealed the kinetics, and … peterhof interior